??>?? ce????b?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ?R??.bjbj  2D|f1g|f1g.%?????????.....????BBB8zT?lBbF?::"\\\????E?E?E?E?E?E?E$?H??Kr?E9.??????E..\\4F666?6.\.\?E6??E66n}=0?B\????`?Z??????????@ ?E2F0bF?@?LT??L@?B?BL??L$.?B???6??????E?E?H???bF?????????????????????????????????????????????????????????????????????????L??????????b ?:  -N?V?QN'Yf[ ,g?yu URP ?zy?3u??fN y??v T?y \??ag??u\o??g?IQ1?e?a?vKm 3u ?? ?N l?`S?? ?]\OUSMO iir?O?bf[b? T??|5u? 010-62733008 5uP[?O?{ mazh@cau.edu.cn kXh??eg 2020.12.8 -N?V?QN'Yf[Ye?RY6R y??v T?y\??ag??u\o??g?IQ1?e?a?vKmy??vw?bk?e?2020.12-2021.12y??v3u???N?`?Q?Y Tl?`S??'`+R7u?Qu?eg1964 . 07gTf[?SNf[MOxvzu ?ZS?XNN?b/gL??RYe?c?N?NNN iir?utf[y??v?i?Q ?vMR)R(u?IQ1?e?a?b/g(W\??ag??u?v?vKm?eb??]?~?S?_OY>fW?'`?vb?g??(gfI{xvzh?f ?(W?Q\??ag??u?Su?eg ?)R(u?IQ1?e?a?b/g?S?N??u?[ZP?Q??e??(gfI{ ?2003 ?0 _?s ?I{?ǐ0u??N?]?c?y\??ag??u ?~b?Q?NIQ1?pencN?u?`cpe(DI)X[(W?vsQ'`?v?l?k??V ??g?^?N\?? N*Nu??g?v?QB\IQ1?pencNvQ?[?^?v?u?`cpe?v?VR_?e z? _?s ?I{ ?2007 ?0FO/f?N6q@\P?(W?u?`cpeN?IQ1? i??cpe?v?[?^0XoZZI{?2010 ??????Q?[\??ag??yr_'`?+R?v_irbeta f/beta r ??^?z?NReal-time PCR ?vKm?[?e?l ??S?[?c?yT?v\???SGr-Nag??DNA ?v+T??L??[?Km?[ ??:N\??ag??u(W\o??g?v?S?e0?_??vKm?c?O?N?Onc0?sOOI{?2011 ?xvzh?f ?Real-time PCR?e?l?S?N?[\??ag??u(W\o??g?v?O?g z?^?L??0O ?RP[?u?`cpe(MDI) NDI KN?X[(W?g>fW??v?vsQ'`0_NsS?f ??S?N?ǐMDI?[0u??v?[E??S?u?`?Q?L???Km0 ?\o??g? iir-N?v?N"??SS?S?N?ǐ?IQ1?b?Peg?hKm(Arens et al, 2016) ??Vdk ? g?S???ǐ N T:\?^ N?v?IQ1?b?Peg?hKm?T?+R?u?ur?S?T?R`(Bravo et al., 2004?Huang et al., 2007)0?vMR,(W\o??g)R(u?IQ1?e?a?vKm\??ag??u MDI ?N Nb?q ?,gxvz?e(W?Nek?g?^?vsQ!j?W0?N0?[?e??R?S?eHh(fnxT*N?s???[?W{Qf[uR?e???R?vwQSO`??S?8h?R?l) 102020.12-2021.05 ??[Y*N N T?b'`\???T?y?L??[?Q?c?y ?v^?[g?L?MDIKm?[?N?SUS*h?IQ1?Km?[*,.0246>@P^`fjlr???????????????????ù??????v??j?_????Q?_???h1q?hx,?5?>*CJ PJh,}!5?>*CJ PJh,}!5?>*CJ PJo(h1q?h?i)5?CJ PJh1q?h ?5?CJ PJo(hF ThF T5?>*CJ PJo(h1q?h ?5?>*CJ PJh1q?h ?5?CJ PJh1q?h?i)5?CJ,h1q?h ?5?CJ,h1q?h ?5?CJ4PJh1q?h?i)5?CJ4h1q?h ?5?CJ4h1q?h ?5?CJ4PJ h1q?h ?.0246`?? > | ? ? ? ? ? ? ???????????????????$a$gd ?gd ??f??d??VDY]?f?^?gd ???d??VDY]?^?gd7;? $?]?a$gd ?$ ?X ?]?a$gd ? $?r]?ra$gd ???????     , 4 6 8 < > F H L P X Z ^ l v z | ? ? ? ? ? ? ? ? ? ? ? ? ? ? ???????????????????????????????}}?vvvnh??h??o( h1q?h ?h7;?5?CJ PJaJ h1q?h ?5?CJ PJaJ h1q?h ?5?h1q?h ?5?PJh??5?>*CJ PJh1q?h?&?5?>*CJ PJh,}!5?>*CJ PJo(h1q?h ?5?CJ PJh,}!5?>*CJ PJh1q?hE 45?>*CJ PJh1q?h ?5?>*CJ PJ*? ? ? ? ? ? ? ? ?????_?V $$Ifa$vkd$$If???0%X ?%3?0???????X ??????????????4?4? a? $$Ifa$gdc? $$Ifa$ $ ?A?^?a$$a$gd ?? ? ? ? ? ? ? ?       " $ * , 2 4 6 @ D H J L N R T V f r t ? ? ? ? ? ? ? ? ? ? > J R V r z ? ? ? ? ? ? ???????????????????????????????????????????????????????h?#?h3?KHaJh3?KHaJh3?KHOJ^JaJo(h1q?h ?aJh1q?h ?o(h?:? h?:?o( h1q?h?]?h?? h1q?h?!?h1q?h ?CJKHaJ h1q?h ?h??h??o( hF To(9  ?? $$Ifa$vkd?$$If???0%X ?%3?0???????X ??????????????4?4? a?  $ , 2 6 @ T ??????? $$Ifa$fkd6$$If?4??X ?X ?0???????X ??????????4?4? a?f4T V f t ? ? 8//// $$Ifa$?kd?$$If?4??C??? ???X ???D?????d?d?0???????X ??????????????????????????????4?4? a?f4? ? ? ? ? ??/% & F$If?kd?$$If?4??B??? ???X ???D?????d?d?0???????X ??????????????????????????????4?4? a?f4 $$Ifa$? ?    ( . 2 4 > F Z j r t ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?  & ( 0 2 < V ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? &L???????????????????????????????????????????????????????h?^UhF TKHaJhF ThF TKHOJ^JaJo(hF TKHOJ^JaJo(h3?KHOJ^JaJh6Q?KHOJ^JaJh?^Uh3?KHaJh?#?h3?KHaJh3?KHaJh3?KHOJ^JaJo(:L???????????  ,8:<>BPRVz??????????????????????????????yr?i`W`h?V?h??aJh?V?h?.aJh1q?h?uaJ h1q?hxh? h1q?hhZ?h1q?hhZ?aJh1q?h ?aJh1q?h ?KHaJh1q?h ?5?aJhq?KHOJ^JaJo(h??h?Q)KHaJh??h??KHaJhF TKHOJ^JaJo(h?Q)KHOJ^JaJo(h?^UhF TKHaJhF ThF TKHOJ^JaJo("? <>??*?+?+??reV??$IfWD?`??gdM? ??$If`??gd? r ??$If`??gd#$Ifckdg$$If???X X ?0???????X ??????????4?4? a?$??$7$8$H$IfWD?`??a$gdF T???????????????????*** *(*,*4*8*:*<*@*D*J*N*R*V*`*b*n*p*|*~*?*?*?*?*?*?*?*?*?*?*?*?*?*?*?*?*?*?????????????????ο??????????????????????????????????????h1q?hc?aJh1q?haJo( h?s?aJ h?s?aJo( h?K?aJo( hF?aJU hF?aJo( h?#(aJ h?Q?aJo( h?#(aJo(h1q?h#aJh1q?h?.aJh?V?h?.aJh?V?h??aJ9 ?6e??vsQpenc0?['Y0u?y i?vY*N N T?b'`\???T?y?L??c?y ?v^?[g?L?MDIKm?[?N?S?QB\?IQ1?Km?[ ?6e??vsQpenc0(W?Nǐ z-N ??8hbXT?v\??ag??A~??d\O0?IQ1??NhV?vO(u?N?SReal-time PCR?NhV?vO(u?v?q?~ z?^0 202021.05-.2021.08 ?pencYt6??k0\O(uPython0Tensorflow0KreasI{?vsQ zo??N ??W?NMRg6e??vpenc ??g?^ i??cpe ??^?z?vsQ!j?W?N?S????0?8hbXT?[pencR?g?e?l?v?c?c?`?Q?N?S z???R?v?NO0  N0y??v?[?e?v?W@x?Tag?N 1.,g?????~?OX[ gǒ????_?v\??ag??u???y ??S(u?N,gy??vxvz? 2.,g?????~wQ g?W{Q\???Tag??u??v?N?]lP?[0 3.,g?????~(W?vsQxvz?eb?wQ gN?[?v?y/}?T?~??0?V0f[u?c?N?vb?g 10US*h4ls^?N?S?QB\4ls^?v?IQ1??vKmpenc0MDIKm?[penc0 20?=\?v?[??penc??U_0?S?Ypenc?T?VGr 30URP?~???bJT?  ?N0?~9????{ 10(u?N\???Tag??u??W{Q?v?N?]lP?[?L?5u9??4000CQ 20'Y0u?[??9?(u?1000CQ qQ??5000CQ0 mQ0f[b?URP???[\?~a?? ~{W[ ??v?z ? t^ g ?e      PAGE  PAGE 1 ?*?*?*?*?*?*++++++++(+*+>+@+B+J+P+T+X+l+n+z+?+?+?+?+?+?+?+?+?+?+?+?+?+?+?+???????????????????????ξ???????????{rih1q?h)?aJh1q?haJh1q?h ?aJh1q?h ?KHaJh1q?h)?5?aJh1q?h?E?aJ h$gaJo( hpB?aJo( h#WaJo( h?K?aJo( hD#?aJo(h?V?hn?aJ hn?aJo( h"N?aJo(h1q?h?maJh?V?h#aJh?V?h??aJh?V?h??aJ(?+?+?+(,Z,?,???????$IfWD?`??gdpB? $Ifgd)?ckd?$$If???X X ?0???????X ??????????4?4? a??+?+,,(,Z,?,?,?,?,?,?,?,?,?,?,?,?,?,?,?,?,?,?,?,--- ---- -"-$-&-,-0-2-6-@-F-?????????????????????????????????}ǖrih??OJaJo(h??OJQJaJo(h1q?hJAaJ h1q?h ?h1q?hqRaJh1q?h+)?aJh1q?h)?aJ h??aJ h??aJo(h1q?h? aJh1q?hxh?aJh1q?h ?aJh1q?haJh1q?h ?KHaJh1q?h ?5?aJ hpB?aJo(hpB?hpB?aJo()?,?,?,?,--- -????x????$IfWD?`??gdqR??$IfWD?`??gd?As$Ifckdu$$If???X X ?0???????X ??????????4?4? a? -"-2-n-?-?-?-????????$IfWD?`??gd+)?$Ifckd?$$If???X X ?0???????X ??????????4?4? a?F-j-n-r-~-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-?-`.b.d.f.h.j.l.n.p.r.t.v.x.z.|.~.?.?.?.?.?.?.?.??????????????????????????????????????????????????hzU? hzU?0JjhzU?0JUh`H?jh`H?U h1q?h ?h1q?h ?CJKHaJh1q?h ?5?aJh1q?h ?KHaJh1q?h ?aJh1q?haJ h?DaJ h?DaJo(h1q?h+)?aJh??OJQJaJo(3?-?-?-?-?-?-?-?-?-?-?-?-?-`.b.?????????????? ?:$IfWD4`?:$Ifekd?$$If???? ?X X ?0???????X ??????????4?4? a?b.d.f.j.l.p.r.v.x.|.~.?.?.?.?.?.?.?????????????????h]?h????&`#$ckd$$If???X X ?0???????X ??????????4?4? a??.?.?.?.?.?.?.?.?.?.????????? h1q?h ?h`H?hzU?h>0JmHnHujhzU?0JU hzU?0J ?.?.?.??01?82P??. ??A!?"?#??$??%??S?? ???$$If?!vh#v%#v3:V ??0???????X ?,?5?%5?3/? ?4? a??$$If?!vh#v%#v3:V ??0???????X ?,?5?%5?3/? ?4? a??$$If?!vh#vX :V ?4?0???????X ?,?5?X /? ?4? a?f4?$$If?!vh#v?#vD#v?#v?#vd:V ?4?C?0???????X ?,?5??5?D5??5??5?d/? ?4? a?f4?$$If?!vh#v?#vD#v?#v?#vd:V ?4?B?0???????X ?,?5??5?D5??5??5?d/? ?4? a?f4?$$If?!vh#vX :V ??0???????X ?5?X /? ?4? a??$$If?!vh#vX :V ??0???????X ?5?X /? ?4? a??$$If?!vh#vX :V ??0???????X ?5?X /? ?4? a??$$If?!vh#vX :V ??0???????X ?5?X /? ?4? a??$$If?!vh#vX :V ??? ?0???????X ?5?X /? ?4? a??$$If?!vh#vX :V ??0???????X ?5?X /? ?4? a?????i???[?C????i???[?C????i???[?C????i???[?C????i???[?C????i???[?C????i???[?Cx2????&6FVfv??????2(??&6FVfv??????&6FVfv??????&6FVfv??????&6FVfv??????&6FVfv??????&6FVfv??8X?V~???????? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@?? 0@66666PJ_HmH nHsH tHJ`??J ck?e $1$a$ CJKH_HaJmH nHsH tH$A`???$ ????k=?W[SOFi@???F nf?h????C?????v?U?A ??6F?????????n?|Q?sAX\?? ???>?8?????O????( ?b\?'X?77????!????@5??2?XY}XF?Kp ???GH?%V???? ?????b?????????|???G_???9??`(?B-?)?( ??????b"?{G??|07`?]?P?EB??_??????1?r??!???S???`??m?-???V?_?\???J???????NS.???j?4???Cw??\+ZxC??筲?????/-???x??kP?//????z??A)?????????_?BJ???P??5f?? CF????r?]]?*?Q? Yv)C?e??C??P@?$?=9I??!?_???????vHB?%(fV ??va ??????602?- "?O?9Id? Z}r?????'?=y?????S?Z?%?????{??W?<????wo?~???? om?v?{???_?8}???:?oq?3?]a???c?.?`???/&? 1%??@?)+?-Z? ???c??9T????"? ?X??;adw?u?^??ln???m??M?]??\?(???'Pb?Ke#???b?c?{l??cw???K?? 6????q??KzV6?B?$??L\!?ov?{uF]?n?# ???]L-7?Bc?"??.????$C???M\KH?t?)?Z,?Kf??~???AP?a???FrIF.?;?1?d?F????84????(??t?w????k?????>?V???????Qw???[?Y???!?R??#??nq???8?R{c???c??u?X>?I???lcWb?Fv??? ???f?N?a?ll ???\?8B|??=????V??????>?L??I???}:??^?? DVS???n?? ?L?D??CY,XW#??H???G?e??? ?l?d|0??????d???u???C?h??P??w?>??uf?E%??=qv???Q?90`????ǚ???????R???@x pf???????]6H?:?Eu??vV?d?^(g?pL%St???e?5?@??(??0??$?$??E?$}? Ns??;{/,?,?+??P-?jYT?????k~?" ?q0?T??P????>#??V??=??λZ??`%???N88?zt??"H?r??8 ^So? ? Y?s?iZv?wQ:??uD?M;?Y?S?.?}??????j?d?{?j??S?n?u????{???Q4?iU?5?U?0ks??\?7X?\ 5???i?/??Z77'd]????u?? j?1??b?X?U???c??3???IU?2S;緬G8???:???g-, gs???~un??f?C(M?r?T Jxi? D=?deC?n? ??PK! ???'theme/theme/_rels/themeManager.xml.rels??M ?0???woo??&?Э???5 6?$Q?? ?,.?a??i????c2?1h?:?q??m??@RN??;d?`??o7?g?K(M&$R(.1?r'J???T???8??V?"??A??H?u}??|?$?b{??P????8?g/]?QAs?(????#??L?[??????PK-!????[Content_Types].xmlPK-!????6 0_rels/.relsPK-!ky???theme/theme/themeManager.xmlPK-!?R???theme/theme/theme1.xmlPK-! ???'? theme/theme/_rels/themeManager.xml.relsPK]? T.D???? $$$'?? ? L??*?+F-?.?.  ?  T ? ? ?+?, -?-b.?.?. !  '!?!t????@? @?????????H ??0?( ? ????0?( ? ??B ?S ???? ?? 0145LPQWdh???????????? +239:@ACDHINPTU}????????$-BCTWlw??????????&GZ[nors???????????? "`emn~?????????'*@BKMNdhkl????????????????????????? $%)*.0134679:RU &??BH??.0134679:RU333333????? +AINU~?.0134679:RU /??Uoqv??????:<FHej?????????@AANh??????-..01134679:RU ?TQ-??????????]??| ????????? &x?2)??????????|?'? NG??????????l?0L????????????? a1t???????????|^K4????????????)drA?F9??????????NBj????????????%?K.????????????`[?J???????????lJ????????????F)?ub??d????????? ???\???^??`?\?0???????^??`???.?p????p^?p`???.?@ ????@ ^?@ `???.?????^?`???.???????^??`???.???????^??`???.???????^??`???.?P????P^?P`???.?h????h^?h`???o(0??H?\??H^?H`?\?)????\???^??`?\?.????\???^??`?\?.??4?\??4^?4`?\?)??? ?\??? ^?? `?\?.??| ?\??| ^?| `?\?.?? ?\?? ^? `?\?)????\???^??`?\?.???\???^??`?\?OJ QJ o(?h?Hl???d?\??d^?d`?\?OJ QJ o(?h?Hn????\??^?`?\?OJ QJ o(?h?Hu?????\???^??`?\?OJ QJ o(?h?Hl???P ?\??P ^?P `?\?OJ QJ o(?h?Hn???? ?\??? ^?? `?\?OJ QJ o(?h?Hu???? ?\??? ^?? `?\?OJ QJ o(?h?Hl???<?\??<^?<`?\?OJ QJ o(?h?Hn?????\???^??`?\?OJ QJ o(?h?Hu??h????h^?h`???.???????^??`???.?p????p^?p`???.?@ ????@ ^?@ `???.?????^?`???.???????^??`???.???????^??`???.???????^??`???.?P????P^?P`???.?h????h^?h`???0???????^??`???.?p????p^?p`???.?@ ????@ ^?@ `???.?????^?`???.???????^??`???.???????^??`???.???????^??`???.?P????P^?P`???.?h????h^?h`???0???????^??`???.?p????p^?p`???.?@ ????@ ^?@ `???.?????^?`???.???????^??`???.???????^??`???.???????^??`???.?P????P^?P`???.???\???^??`?\?OJ QJ o(?h?Hl???H?\??H^?H`?\?OJ QJ o(?h?Hn?????\???^??`?\?OJ QJ o(?h?Hu?????\???^??`?\?OJ QJ o(?h?Hl???4?\??4^?4`?\?OJ QJ o(?h?Hn???? ?\??? ^?? `?\?OJ QJ o(?h?Hu???| ?\??| ^?| `?\?OJ QJ o(?h?Hl??? ?\?? ^? `?\?OJ QJ o(?h?Hn?????\???^??`?\?OJ QJ o(?h?Hu????0???^??`?0?? ????????^??`???.?p????p^?p`???.?@ ????@ ^?@ `???.?????^?`???.???????^??`???.???????^??`???.???????^??`???.?P????P^?P`???. ??? ???^??`? ?o(0? ?H?\??H^?H`?\??h?H)? ???\???^??`?\??h?H.? ???\???^??`?\??h?H.? ?4?\??4^?4`?\??h?H)? ?? ?\??? ^?? `?\??h?H.? ?| ?\??| ^?| `?\??h?H.? ? ?\?? ^? `?\??h?H)? ???\???^??`?\??h?H.???????^??`???o(0? ???\???^??`?\??h?H)? ?5?\??5^?5`?\??h?H.? ???\???^??`?\??h?H.? ?} ?\??} ^?} `?\??h?H)? ?! ?\??! ^?! `?\??h?H.? ?? ?\??? ^?? `?\??h?H.? ?i?\??i^?i`?\??h?H)? ? ?\?? ^? `?\??h?H. ?)? ??)^?)`? ?o(0? ???\???^??`?\??h?H)? ?5?\??5^?5`?\??h?H.? ???\???^??`?\??h?H.? ?} ?\??} ^?} `?\??h?H)? ?! ?\??! ^?! `?\??h?H.? ?? ?\??? ^?? `?\??h?H.? ?i?\??i^?i`?\??h?H)? ? ?\?? ^? `?\??h?H.?h????h^?h`???0???????^??`???.?p????p^?p`???.?@ ????@ ^?@ `???.?????^?`???.???????^??`???.???????^??`???.???????^??`???.?P????P^?P`???. ???/???^??`?/?o(0??H?\??H^?H`?\?)????\???^??`?\?.????\???^??`?\?.??4?\??4^?4`?\?)??? ?\??? ^?? `?\?.??| ?\??| ^?| `?\?.?? ?\?? ^? `?\?)????\???^??`?\?.?|?'?|?' ?)drA)drA ??l?0?l?0 ???l??l ?? a1? a1 ??TQ?TQ ?F)?u]?%?K?` &|^K4?NB????????????????? ?5?0????????? ?5?0?????????????????????????????????????????????????????? ?? _?J        4]?#                 2-P?        ?}?E         ??                 T?L:        ?1v?        ?4?        6??        h,?        ???R        4?*????7fF?eK[?%uQh? ?q JA?.?ukZ?D?'/5BA? oC?m?D,}!?g"#?#(b9(?Q)?i)+N,X-?0[z2E 4?H7: 8m,:>z$>c> ?#??AG D.:DTmD?%G}?@ABCDEFGHIJKLMNOPQ????STUVWXY????[\]^_`a????????d????????????????????????????????????????????????????????????????????????????????????????????????????????????????Root Entry???????? ?F?{[???f?Data ????????????#1Table????+*MWordDocument????2DSummaryInformation(????????????RDocumentSummaryInformation8????????ZCompObj????????????n?????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? ???? ?FMicrosoft Word 97-2003 ??? MSWordDocWord.Document.8?9?q